anti- braf v600e mouse monoclonal antibody clone ve1  (Spring Bioscience)

Journal: Annals of Surgical Oncology

Article Title: Quantitative and Spatial Analysis of CD8+/PD-1 Tumor-Infiltrating Lymphocytes as a Predictive Biomarker for Clinical Response of Melanoma In-Transit Metastases to Topical Immunotherapy

doi: 10.1245/s10434-020-08713-1

Figure Lengend Snippet: Lymphocyte biomarkers and distribution by BRAF mutation status

Article Snippet: For BRAF V600E IHC, Pleasanton, CA Spring Bioscience mouse anti-human BRAF V600E monoclonal antibody (Clone VE1) was used at a dilution of 1:200 run on a routine long program.

Techniques: Mutagenesis

Journal: Annals of Surgical Oncology

Article Title: Quantitative and Spatial Analysis of CD8+/PD-1 Tumor-Infiltrating Lymphocytes as a Predictive Biomarker for Clinical Response of Melanoma In-Transit Metastases to Topical Immunotherapy

doi: 10.1245/s10434-020-08713-1

Figure Lengend Snippet: Clinical response to DPCP correlated with OS by biomarker status

Article Snippet: For BRAF V600E IHC, Pleasanton, CA Spring Bioscience mouse anti-human BRAF V600E monoclonal antibody (Clone VE1) was used at a dilution of 1:200 run on a routine long program.

Techniques: Biomarker Discovery

Journal: Histopathology

Article Title: BRAF V600E mutations and immunohistochemical expression of VE1 protein in low-grade serous neoplasms of the ovary

doi: 10.1111/his.13651

Figure Lengend Snippet: Examples of serous borderline tumour with eosinophilic cells and budding (A, B), with cytoplasmic staining for VE1 (C, D). Haematoxylin-eosin stain (A, B); VE1 immunohistochemical stain (C, D). Original magnification ×100 (A, C); ×200 (B, D).

Article Snippet: Immunohistochemistry was performed on 4-micron thick archival formalin-fixed paraffin-embedded (FFPE) tissue sections using the mouse monoclonal anti- BRAF V600E antibody, clone VE1 (dilution 1:800, catalogue number E19294, Spring Bioscience, Pleasanton, CA, USA) on a Bond III automated staining system (Leica Biosystems, Buffalo Grove, IL, USA) according to the manufacturer’s instructions.

Techniques: Staining, Immunohistochemical staining

Journal: Histopathology

Article Title: BRAF V600E mutations and immunohistochemical expression of VE1 protein in low-grade serous neoplasms of the ovary

doi: 10.1111/his.13651

Figure Lengend Snippet: Serous borderline tumour (A) associated with low-grade serous carcinoma (B), both tumours exhibiting cytoplasmic expression of VE1 (C and D, respectively). Haematoxylin-eosin stain (A, B); VE1 immunohistochemical stain (C, D). Original magnification ×100 (A, C); ×400 (B, D).

Article Snippet: Immunohistochemistry was performed on 4-micron thick archival formalin-fixed paraffin-embedded (FFPE) tissue sections using the mouse monoclonal anti- BRAF V600E antibody, clone VE1 (dilution 1:800, catalogue number E19294, Spring Bioscience, Pleasanton, CA, USA) on a Bond III automated staining system (Leica Biosystems, Buffalo Grove, IL, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: Histopathology

Article Title: BRAF V600E mutations and immunohistochemical expression of VE1 protein in low-grade serous neoplasms of the ovary

doi: 10.1111/his.13651

Figure Lengend Snippet: BRAF mutation-associated histological features, VE1 immunohistochemistry and mutation frequencies in 76 tumours with known mutation status (all p <0.0001)

Article Snippet: Immunohistochemistry was performed on 4-micron thick archival formalin-fixed paraffin-embedded (FFPE) tissue sections using the mouse monoclonal anti- BRAF V600E antibody, clone VE1 (dilution 1:800, catalogue number E19294, Spring Bioscience, Pleasanton, CA, USA) on a Bond III automated staining system (Leica Biosystems, Buffalo Grove, IL, USA) according to the manufacturer’s instructions.

Techniques: Mutagenesis, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: BRAF V600E Inhibitor (Vemurafenib) for BRAF V600E Mutated Low Grade Gliomas

doi: 10.3389/fonc.2018.00526

Figure Lengend Snippet: Clinical-pathological characteristics of patients and BRAF V600E immunostain intensity.

Article Snippet: As for immunohistochemistry, 3-micron paraffin embedded sections were dehydrated, pretreated with avidin block for 15' and biotin block for 15', then incubated with BSA for 30' and incubated with mouse anti-human BRAF V600E monoclonal antibody (clone VE1, Spring Bioscience) at room temperature for 60' (dilution 1:50, PT-link antigen retrival at high pH).

Techniques: Sequencing